southern-blot与northern-blot的区别都有哪些具体的区别

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southern-blot与northern-blot的区别都有哪些具体的区别

southern-blot与northern-blot的区别都有哪些具体的区别
southern-blot与northern-blot的区别
都有哪些具体的区别

southern-blot与northern-blot的区别都有哪些具体的区别
A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples.
The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.

最本质的区别是前者是DNA和DNA的杂交,后者是DNA和RNA杂交。

Southern blot 是分析DNA的杂交技术,Northern blot是分析RNA的,而Western 和Northerin原理基本相同,都是利用DNA或RNA的复性过程,但过程上也有区别,